Excision of Transducing phages in Lambdoid phages
نویسندگان
چکیده
منابع مشابه
Transducing phages for Bacillus subtilis.
Transducing phage PBSl and a clear-plaque type mutant PBS2 derived from PBS 1 show similar growth characteristics with a relatively small burst size (23 to 24), and are related serologically. They are morphologically indistinguishable and both have a dense head of about 100 mp in diameter and a tail about 250 m p in length. PBS 1 is more efficient in transduction and its plaque-forming activity...
متن کاملComparative molecular biology of lambdoid phages.
Lambdoid phages are natural relatives of phage lambda. As a group, they are highly polymorphic in DNA sequence and biological specificity. Specificity differences have played a key role in identifying the specific sequences recognized by the N and Q antitermination proteins, the initiator O for DNA synthesis, the terminase system Nv1-A for cutting DNA during packaging, and the cI repressor prot...
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Salmonella phage P22 has been used in the construction of three sorts of specialized transducing phage: P22 proAB, P22 proABlac and P22 argF. The bacterial genes carried are derived from E. coli K12. Since E. coli and Salmonella chromosomes recombine very poorly, E. coli genes cannot be transduced into Salmonella recipients by P22‘s generalized transduction mechanism. Therefore, stable inherita...
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We studied the role of DNA ligase in illegitimate recombination in Escherichia coli. A temperature-sensitive mutation in the lig gene reduced the frequency with which lambdabio-transducing phages were generated to 10-14% of that of wild type under UV irradiation. Reintroduction of the lig gene into this mutant restored the frequency of recombinant phage generation to that of wild type. Furtherm...
متن کاملPhages in the Human Body
Bacteriophages, viruses that infect bacteria, have re-emerged as powerful regulators of bacterial populations in natural ecosystems. Phages invade the human body, just as they do other natural environments, to such an extent that they are the most numerous group in the human virome. This was only revealed in recent metagenomic studies, despite the fact that the presence of phages in the human b...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1976
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.16.278